Western Blot:Article Title: Asparagine Endopeptidase Inhibition Attenuates Tissue Plasminogen Activator‐Induced Brain Hemorrhagic Transformation After Ischemic Stroke
Article Snippet: The following antibodies and reagents were used: Legumain (AEP) (#93627, Cell Signaling Technology, 1:1000 for western blotting and 1:500 for immunofluorescence), ZO‐1 (YN1410, Immunoway, 1:1000 for western blotting), claudin‐5 (YT0953, Immunoway, 1:1000 for western blotting), occludin (YN2865, Immunoway, 1:1000 for western blotting), JAM‐1 (YT5479, Immunoway, 1:1000 for western blotting), Collagen IV (YM3756, Immunoway, 1:500 for immunofluorescence), CD31 (YM6115, Immunoway, 1:500 for immunofluorescence), LRP‐1 (#64099, Cell Signaling Technology, 1:500 for western blotting), MMP2 (YT2798, Immunoway, 1:1000 for western blotting), MMP3 (YT4465, Immunoway, 1:1000 for western blotting), MMP9 (YT1892, Immunoway, 1:1000 for western blotting), phospho‐TrkB (#4621, Cell Signaling Technology, 1:500 for western blotting), TrkB (#4603, Cell Signaling Technology, 1:500 for western blotting), β‐Actin (#4970, Cell Signaling Technology, 1:2000 for western blotting), anti‐rabbit IgG‐HRP (#70745, Cell Signaling Technology, 1:3000 for western blotting), Alexa Fluor 594‐conjugated goat anti‐mouse IgG (Invitrogen, A‐11005, 1:1000 for immunofluorescence), Alexa Fluor 488‐conjugated goat anti‐rabbit IgG (Invitrogen, A‐32731, 1:1000 for immunofluorescence), DAPI (C1002, Beyotime), TUNEL cell death detection kit (C1090, Beyotime), 2,3,5‐Triphenyl‐2H‐Tetrazolium Chloride (TTC) (G3005, Solarbio), Evans Blue Solution (E8010, Solarbio), Hematoxylin Staining Solution (C0170, Beyotime), Eosin Staining Solution (C0190, Beyotime), Cell Counting Kit‐8 (C0037, Beyotime).
Immunofluorescence:Article Title: Asparagine Endopeptidase Inhibition Attenuates Tissue Plasminogen Activator‐Induced Brain Hemorrhagic Transformation After Ischemic Stroke
Article Snippet: The following antibodies and reagents were used: Legumain (AEP) (#93627, Cell Signaling Technology, 1:1000 for western blotting and 1:500 for immunofluorescence), ZO‐1 (YN1410, Immunoway, 1:1000 for western blotting), claudin‐5 (YT0953, Immunoway, 1:1000 for western blotting), occludin (YN2865, Immunoway, 1:1000 for western blotting), JAM‐1 (YT5479, Immunoway, 1:1000 for western blotting), Collagen IV (YM3756, Immunoway, 1:500 for immunofluorescence), CD31 (YM6115, Immunoway, 1:500 for immunofluorescence), LRP‐1 (#64099, Cell Signaling Technology, 1:500 for western blotting), MMP2 (YT2798, Immunoway, 1:1000 for western blotting), MMP3 (YT4465, Immunoway, 1:1000 for western blotting), MMP9 (YT1892, Immunoway, 1:1000 for western blotting), phospho‐TrkB (#4621, Cell Signaling Technology, 1:500 for western blotting), TrkB (#4603, Cell Signaling Technology, 1:500 for western blotting), β‐Actin (#4970, Cell Signaling Technology, 1:2000 for western blotting), anti‐rabbit IgG‐HRP (#70745, Cell Signaling Technology, 1:3000 for western blotting), Alexa Fluor 594‐conjugated goat anti‐mouse IgG (Invitrogen, A‐11005, 1:1000 for immunofluorescence), Alexa Fluor 488‐conjugated goat anti‐rabbit IgG (Invitrogen, A‐32731, 1:1000 for immunofluorescence), DAPI (C1002, Beyotime), TUNEL cell death detection kit (C1090, Beyotime), 2,3,5‐Triphenyl‐2H‐Tetrazolium Chloride (TTC) (G3005, Solarbio), Evans Blue Solution (E8010, Solarbio), Hematoxylin Staining Solution (C0170, Beyotime), Eosin Staining Solution (C0190, Beyotime), Cell Counting Kit‐8 (C0037, Beyotime).
TUNEL Assay:Article Title: Asparagine Endopeptidase Inhibition Attenuates Tissue Plasminogen Activator‐Induced Brain Hemorrhagic Transformation After Ischemic Stroke
Article Snippet: The following antibodies and reagents were used: Legumain (AEP) (#93627, Cell Signaling Technology, 1:1000 for western blotting and 1:500 for immunofluorescence), ZO‐1 (YN1410, Immunoway, 1:1000 for western blotting), claudin‐5 (YT0953, Immunoway, 1:1000 for western blotting), occludin (YN2865, Immunoway, 1:1000 for western blotting), JAM‐1 (YT5479, Immunoway, 1:1000 for western blotting), Collagen IV (YM3756, Immunoway, 1:500 for immunofluorescence), CD31 (YM6115, Immunoway, 1:500 for immunofluorescence), LRP‐1 (#64099, Cell Signaling Technology, 1:500 for western blotting), MMP2 (YT2798, Immunoway, 1:1000 for western blotting), MMP3 (YT4465, Immunoway, 1:1000 for western blotting), MMP9 (YT1892, Immunoway, 1:1000 for western blotting), phospho‐TrkB (#4621, Cell Signaling Technology, 1:500 for western blotting), TrkB (#4603, Cell Signaling Technology, 1:500 for western blotting), β‐Actin (#4970, Cell Signaling Technology, 1:2000 for western blotting), anti‐rabbit IgG‐HRP (#70745, Cell Signaling Technology, 1:3000 for western blotting), Alexa Fluor 594‐conjugated goat anti‐mouse IgG (Invitrogen, A‐11005, 1:1000 for immunofluorescence), Alexa Fluor 488‐conjugated goat anti‐rabbit IgG (Invitrogen, A‐32731, 1:1000 for immunofluorescence), DAPI (C1002, Beyotime), TUNEL cell death detection kit (C1090, Beyotime), 2,3,5‐Triphenyl‐2H‐Tetrazolium Chloride (TTC) (G3005, Solarbio), Evans Blue Solution (E8010, Solarbio), Hematoxylin Staining Solution (C0170, Beyotime), Eosin Staining Solution (C0190, Beyotime), Cell Counting Kit‐8 (C0037, Beyotime).
Staining:Article Title: Asparagine Endopeptidase Inhibition Attenuates Tissue Plasminogen Activator‐Induced Brain Hemorrhagic Transformation After Ischemic Stroke
Article Snippet: The following antibodies and reagents were used: Legumain (AEP) (#93627, Cell Signaling Technology, 1:1000 for western blotting and 1:500 for immunofluorescence), ZO‐1 (YN1410, Immunoway, 1:1000 for western blotting), claudin‐5 (YT0953, Immunoway, 1:1000 for western blotting), occludin (YN2865, Immunoway, 1:1000 for western blotting), JAM‐1 (YT5479, Immunoway, 1:1000 for western blotting), Collagen IV (YM3756, Immunoway, 1:500 for immunofluorescence), CD31 (YM6115, Immunoway, 1:500 for immunofluorescence), LRP‐1 (#64099, Cell Signaling Technology, 1:500 for western blotting), MMP2 (YT2798, Immunoway, 1:1000 for western blotting), MMP3 (YT4465, Immunoway, 1:1000 for western blotting), MMP9 (YT1892, Immunoway, 1:1000 for western blotting), phospho‐TrkB (#4621, Cell Signaling Technology, 1:500 for western blotting), TrkB (#4603, Cell Signaling Technology, 1:500 for western blotting), β‐Actin (#4970, Cell Signaling Technology, 1:2000 for western blotting), anti‐rabbit IgG‐HRP (#70745, Cell Signaling Technology, 1:3000 for western blotting), Alexa Fluor 594‐conjugated goat anti‐mouse IgG (Invitrogen, A‐11005, 1:1000 for immunofluorescence), Alexa Fluor 488‐conjugated goat anti‐rabbit IgG (Invitrogen, A‐32731, 1:1000 for immunofluorescence), DAPI (C1002, Beyotime), TUNEL cell death detection kit (C1090, Beyotime), 2,3,5‐Triphenyl‐2H‐Tetrazolium Chloride (TTC) (G3005, Solarbio), Evans Blue Solution (E8010, Solarbio), Hematoxylin Staining Solution (C0170, Beyotime), Eosin Staining Solution (C0190, Beyotime), Cell Counting Kit‐8 (C0037, Beyotime).
CCK-8 Assay:Article Title: Asparagine Endopeptidase Inhibition Attenuates Tissue Plasminogen Activator‐Induced Brain Hemorrhagic Transformation After Ischemic Stroke
Article Snippet: The following antibodies and reagents were used: Legumain (AEP) (#93627, Cell Signaling Technology, 1:1000 for western blotting and 1:500 for immunofluorescence), ZO‐1 (YN1410, Immunoway, 1:1000 for western blotting), claudin‐5 (YT0953, Immunoway, 1:1000 for western blotting), occludin (YN2865, Immunoway, 1:1000 for western blotting), JAM‐1 (YT5479, Immunoway, 1:1000 for western blotting), Collagen IV (YM3756, Immunoway, 1:500 for immunofluorescence), CD31 (YM6115, Immunoway, 1:500 for immunofluorescence), LRP‐1 (#64099, Cell Signaling Technology, 1:500 for western blotting), MMP2 (YT2798, Immunoway, 1:1000 for western blotting), MMP3 (YT4465, Immunoway, 1:1000 for western blotting), MMP9 (YT1892, Immunoway, 1:1000 for western blotting), phospho‐TrkB (#4621, Cell Signaling Technology, 1:500 for western blotting), TrkB (#4603, Cell Signaling Technology, 1:500 for western blotting), β‐Actin (#4970, Cell Signaling Technology, 1:2000 for western blotting), anti‐rabbit IgG‐HRP (#70745, Cell Signaling Technology, 1:3000 for western blotting), Alexa Fluor 594‐conjugated goat anti‐mouse IgG (Invitrogen, A‐11005, 1:1000 for immunofluorescence), Alexa Fluor 488‐conjugated goat anti‐rabbit IgG (Invitrogen, A‐32731, 1:1000 for immunofluorescence), DAPI (C1002, Beyotime), TUNEL cell death detection kit (C1090, Beyotime), 2,3,5‐Triphenyl‐2H‐Tetrazolium Chloride (TTC) (G3005, Solarbio), Evans Blue Solution (E8010, Solarbio), Hematoxylin Staining Solution (C0170, Beyotime), Eosin Staining Solution (C0190, Beyotime), Cell Counting Kit‐8 (C0037, Beyotime).
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